Kumulative dissertation umfang
First, a novel label-free P.
This invasive plant pathogen was successfully detected and the entire analysis chain including, sample preparation, DNA isolation, amplification and hybridization on SERS-active silver substrate-immobilized adenine-free capture probes is introduced in the thesis.
These procedure increased their sensitivity, specificity and shelf-life.
Second, hierarchically designed 3D flower-like hybrid nanostructures were developed by combining the metastable property of EGNPs substrates with plasma enhanced atomic layer deposition PE-ALD.
The resulting ultra-stable nanostructures were established as an efficient SERS-template substrate.
Finally, an alternative silver nanoparticle synthesizing method using microwave radiation was proposed. This employs as a reliable, rapid, simple, reproducible and environmental friendly bottom-up fabrication strategy. The approach was implemented to the glass capillaries.
For this kumulative dissertation umfang, not only the physical, material and optical properties of these developed nanostructures were characterized but also their SERS efficiency was studied in detail. The developed novel SERS-active nanostructures show improved SERS performance in terms of high SERS reproducibility, surface uniformity, easy synthesis, cost-effectiveness, easy handling and high signal to noise ratio.
Finally, the great potential of those novel SERS-active substrates in the field of biochemical and trace detection of an illegal drug were highlighted.
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